Journal: The Journal of Biological Chemistry

Article Title: Human Memory, but Not Naive, CD4 + T Cells Expressing Transcription Factor T-bet Might Drive Rapid Cytokine Production *

doi: 10.1074/jbc.M114.608745

Figure Lengend Snippet: Kinetics of cytokine secretion by activated naive and memory CD4+ T cells. Naive and memory CD4+ T cells were isolated from CD4+ T cells by MACS microbeads, and the purity of naive or memory CD4+ T cells was more than 97% as determined by flow cytometry (a). Naive and memory CD4+ T cells were stimulated with (open histogram) or without (shaded histogram) PMA and ionomycin in the presence of BFA for 6 h. Cells were stained by intracellular staining and analyzed by FACS (b). Purified naive and memory CD4+ T cells were stimulated with immobilized anti-CD3 (1 μg/ml) and anti-CD28 (1 μg/ml) for 0 to 72 h, and the concentrations of cytokines IFN-γ, IL-2, and TNF-α were detected by ELISA (c). Data are representative of three separate experiments with similar results.

Article Snippet: Human ELISA kits for cytokines IFN-γ, IL-2, and TNF-α were purchased from BD Biosciences.

Techniques: Isolation, Flow Cytometry, Staining, Purification, Enzyme-linked Immunosorbent Assay

Journal: Oncotarget

Article Title: Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer in urine samples

doi: 10.18632/oncotarget.9406

Figure Lengend Snippet: Highly concordant results between replicate measurements of AIB1 ( A1 , B1 ), EIF5A2 ( A2 , B2 ), and NMP22 ( A3 , B3 ) were obtained in the training and independent validation sets.

Article Snippet: ELISA kits of human AIB1 (SEE798Hu) and human NMP22 (NUMA1: SEC332Hu) were purchased from USCN Life Science (Wuhan, China).

Techniques: Biomarker Discovery

Journal: Oncotarget

Article Title: Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer in urine samples

doi: 10.18632/oncotarget.9406

Figure Lengend Snippet: The protein levels of AIB1 ( A1 , B1 ), EIF5A2 ( A2 , B2 ), and NMP22 ( A3 , B3 ) are substantially elevated in BCa urine samples when compared with the non-cancer urine samples in the training and independent validation sets ( p < 0.0001 for both). The results in all groups are presented as the mean and SD. Significance ( p < 0.05) was assessed using the Mann–Whitney U test.

Article Snippet: ELISA kits of human AIB1 (SEE798Hu) and human NMP22 (NUMA1: SEC332Hu) were purchased from USCN Life Science (Wuhan, China).

Techniques: Biomarker Discovery, MANN-WHITNEY

Journal: Oncotarget

Article Title: Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer in urine samples

doi: 10.18632/oncotarget.9406

Figure Lengend Snippet: Association of urinary levels of the three markers with selected patient characteristics

Article Snippet: ELISA kits of human AIB1 (SEE798Hu) and human NMP22 (NUMA1: SEC332Hu) were purchased from USCN Life Science (Wuhan, China).

Techniques: Biomarker Discovery

Journal: Oncotarget

Article Title: Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer in urine samples

doi: 10.18632/oncotarget.9406

Figure Lengend Snippet: Comparisons of the diagnostic performance by the model and individual AIB1, EIF5A2 or NMP22 in the training cohort A. and independent validation set B. .

Article Snippet: ELISA kits of human AIB1 (SEE798Hu) and human NMP22 (NUMA1: SEC332Hu) were purchased from USCN Life Science (Wuhan, China).

Techniques: Diagnostic Assay, Biomarker Discovery

Journal: Oncotarget

Article Title: Identification and validation of AIB1 and EIF5A2 for noninvasive detection of bladder cancer in urine samples

doi: 10.18632/oncotarget.9406

Figure Lengend Snippet: Logistic regression analysis of biomarkers in voided urine of the training and independent validation sets

Article Snippet: ELISA kits of human AIB1 (SEE798Hu) and human NMP22 (NUMA1: SEC332Hu) were purchased from USCN Life Science (Wuhan, China).

Techniques: Biomarker Discovery